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(A) IC 50 results of DOX·HCl、DOX/VCPH、DOX/VCPH/pDNA nanoparticles with different DOX concentrations against A549 cells. (n = 6) (B) The relative inhibition rate of A549 cells transfected with different nanoparticles (* P < 0.05, n = 6). (C) The Calcein/PI staining images of A549 cells with different nanoparticles. (n = 3) (D) The flow <t>cytometry</t> result of A549 cells with different nanoparticles. (n = 3) (E) GFP-positive rate detected by flow cytometry of A549 cells transfected with different nanocomposite particles(compared with the negative control group, ** P < 0.01, compared with the positive control group, ## P < 0.01, compared between DOX/VCPH/pDNA and VCPH/pDNA, △△ P < 0.01, n = 6). (F) Laser confocal scanning microscopy of A549 cells transfected with different nanocomposite particles. (n = 3).
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(A) IC 50 results of DOX·HCl、DOX/VCPH、DOX/VCPH/pDNA nanoparticles with different DOX concentrations against A549 cells. (n = 6) (B) The relative inhibition rate of A549 cells transfected with different nanoparticles (* P < 0.05, n = 6). (C) The Calcein/PI staining images of A549 cells with different nanoparticles. (n = 3) (D) The flow cytometry result of A549 cells with different nanoparticles. (n = 3) (E) GFP-positive rate detected by flow cytometry of A549 cells transfected with different nanocomposite particles(compared with the negative control group, ** P < 0.01, compared with the positive control group, ## P < 0.01, compared between DOX/VCPH/pDNA and VCPH/pDNA, △△ P < 0.01, n = 6). (F) Laser confocal scanning microscopy of A549 cells transfected with different nanocomposite particles. (n = 3).

Journal: Bioactive Materials

Article Title: STAT3-specific nanocarrier for shRNA/drug dual delivery and tumor synergistic therapy

doi: 10.1016/j.bioactmat.2024.07.010

Figure Lengend Snippet: (A) IC 50 results of DOX·HCl、DOX/VCPH、DOX/VCPH/pDNA nanoparticles with different DOX concentrations against A549 cells. (n = 6) (B) The relative inhibition rate of A549 cells transfected with different nanoparticles (* P < 0.05, n = 6). (C) The Calcein/PI staining images of A549 cells with different nanoparticles. (n = 3) (D) The flow cytometry result of A549 cells with different nanoparticles. (n = 3) (E) GFP-positive rate detected by flow cytometry of A549 cells transfected with different nanocomposite particles(compared with the negative control group, ** P < 0.01, compared with the positive control group, ## P < 0.01, compared between DOX/VCPH/pDNA and VCPH/pDNA, △△ P < 0.01, n = 6). (F) Laser confocal scanning microscopy of A549 cells transfected with different nanocomposite particles. (n = 3).

Article Snippet: After 4 h of cultivation, the complexes were removed and replaced with fresh DMEM, after which the well plates were incubated at 37 °C for another 48 h. The results were obtained using a fluorescence microscope (Leica, Germany) and by using the flow cytometry method (FACE Vantage, BD, USA).

Techniques: Inhibition, Transfection, Staining, Flow Cytometry, Negative Control, Positive Control, Confocal Laser Scanning Microscopy